Research Overview
Key Findings
Developmental Changes
Comprehensive analysis revealed 593 differentially expressed genes between pre-seizure (P15) and seizure-onset (P30) stages in the GAERS model, with 94.4% showing upregulation.
Synaptic Organization
Strong enrichment in synaptic plasticity and postsynaptic density organization pathways, indicating critical circuit refinement during the epileptogenic window.
Calcium Signaling
Dysregulation of calcium homeostasis and ion channel genes, including T-type calcium channels critical for absence seizure generation.
Explore the Data
10X Genomics Spatial Transcriptomics
Explore spatial gene expression patterns in GAERS vs Wistar control rats at postnatal days 15 and 30 using 10X Visium technology.
- 8 spatial samples (P15 & P30)
- 1,079+ differentially expressed genes per comparison
- Cell type-specific markers
- Spatial organization analysis
LCM/Bulk RNA-seq Analysis
Differential expression analysis comparing Ga30 (seizure-onset) vs Ga15 (pre-seizure) developmental stages using laser capture microdissection and bulk RNA-seq.
- 593 significant DEGs
- 560 upregulated, 33 downregulated
- GO & Reactome pathway enrichment
- Interactive visualizations
Interactive Gene Search
Search and filter genes across all datasets. Find genes by symbol, ID, or description with advanced filtering options.
- Search 3,611 genes
- Filter by dataset and regulation
- Sortable results table
- Export to CSV
Methods & Protocols
Detailed documentation of experimental protocols, bioinformatics pipelines, and quality control metrics.
- LCM tissue collection
- 10X Visium workflow
- DESeq2 & Limma analysis
- Enrichment analysis methods
Advanced Spatial Analysis with Loupe Browser
Unlock the full power of spatial transcriptomics
Interactive Spatial Exploration
Download our .cloupe files to explore GAERS spatial transcriptomics data with 10X Genomics Loupe Browser desktop application:
- Query 20,000+ Genes: Visualize any gene's spatial expression pattern
- Interactive Tissue Maps: Overlay gene expression on H&E histology images
- Custom Analysis: Draw regions, perform differential expression, discover clusters
- Publication Quality: Export high-resolution figures for your research
Available Datasets
Before seizure onset (ga2p15_2) - 275 MB
At absence seizure onset (ga1p30_2) - 328 MB
Hybrid Approach: Our website provides fast, pre-computed visualizations for quick exploration. For in-depth spatial analysis, custom gene queries, and publication-ready figures, use Loupe Browser with our downloadable .cloupe files.
About GAERS Model
The Genetic Absence Epilepsy Rats from Strasbourg (GAERS) is a well-established genetic model for studying absence epilepsy. These rats spontaneously develop spike-and-wave discharges (SWDs) characteristic of absence seizures, providing insights into epileptogenesis mechanisms and potential therapeutic targets.